Glembotski lab publications

In chronological order

The peroxisomal enzyme, FAR1, is induced during ER stress in an ATF6-dependent manner in cardiac myocytes.
Although peroxisomes have been extensively studied in other cell types, their presence and function have gone virtually unexamined in …
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Optimizing Adeno-Associated Virus Serotype 9 for Studies of Cardiac Chamber-Specific Gene Regulation.
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Simultaneous Isolation and Culture of Atrial Myocytes, Ventricular Myocytes, and Non-Myocytes from an Adult Mouse Heart.
The isolation and culturing of cardiac myocytes from mice has been essential for furthering the understanding of cardiac physiology and …
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Proteomic analysis of the cardiac myocyte secretome reveals extracellular protective functions for the ER stress response.
The effects of ER stress on protein secretion by cardiac myocytes are not well understood. In this study, the ER stressor thapsigargin …
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Mesencephalic astrocyte-derived neurotrophic factor is an ER-resident chaperone that protects against reductive stress in the heart.
We have previously demonstrated that ischemia/reperfusion (I/R) impairs endoplasmic reticulum (ER)-based protein folding in the heart …
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Reactive Oxygen Species (ROS)-Activatable Prodrug for Selective Activation of ATF6 after Ischemia/Reperfusion Injury.
We describe here the design, synthesis, and biological evaluation of a reactive oxygen species (ROS)-activatable prodrug for the …
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Designing Novel Therapies to Mend Broken Hearts: ATF6 and Cardiac Proteostasis.
The heart exhibits incredible plasticity in response to both environmental and genetic alterations that affect workload. Over the …
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The ER Unfolded Protein Response Effector, ATF6, Reduces Cardiac Fibrosis and Decreases Activation of Cardiac Fibroblasts.
Activating transcription factor-6 α (ATF6) is one of the three main sensors and effectors of the endoplasmic reticulum (ER) stress …
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ATF6 as a Nodal Regulator of Proteostasis in the Heart.
Proteostasis encompasses a homeostatic cellular network in all cells that maintains the integrity of the proteome, which is critical …
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Physiological signaling in the absence of amidated peptides.
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Proteostasis and Beyond: ATF6 in Ischemic Disease.
Endoplasmic reticulum (ER) stress is a pathological hallmark of numerous ischemic diseases, including stroke and myocardial infarction …
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Unfolding the Roles of Mitochondria as Therapeutic Targets for Heart Disease.
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Pharmacologic ATF6 activation confers global protection in widespread disease models by reprograming cellular proteostasis.
Pharmacologic activation of stress-responsive signaling pathways provides a promising approach for ameliorating imbalances in …
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Integrating ER and Mitochondrial Proteostasis in the Healthy and Diseased Heart.
The integrity of the proteome in cardiac myocytes is critical for robust heart function. Proteome integrity in all cells is managed by …
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ATF6 Regulates Cardiac Hypertrophy by Transcriptional Induction of the mTORC1 Activator, Rheb.
RATIONALE: Endoplasmic reticulum (ER) stress dysregulates ER proteostasis, which activates the transcription factor, ATF6 (activating …
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Pharmacologic ATF6 activating compounds are metabolically activated to selectively modify endoplasmic reticulum proteins.
Pharmacologic arm-selective unfolded protein response (UPR) signaling pathway activation is emerging as a promising strategy to …
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ER Protein Quality Control and the Unfolded Protein Response in the Heart.
Cardiac myocytes are the cells responsible for the robust ability of the heart to pump blood throughout the circulatory system. Cardiac …
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Peptidyl-Prolyl Isomerase 1 Regulates Ca(2+) Handling by Modulating Sarco(Endo)Plasmic Reticulum Calcium ATPase and Na(2+)/Ca(2+) Exchanger 1 Protein Levels and Function.
BACKGROUND: Aberrant Ca(2+) handling is a prominent feature of heart failure. Elucidation of the molecular mechanisms responsible for …
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Expanding the Paracrine Hypothesis of Stem Cell-Mediated Repair in the Heart: When the Unconventional Becomes Conventional.
Recent interest in mechanisms of stem cell-mediated repair in the heart have spawned the ``paracrine hypothesis'', which posits that …
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ATF6 Decreases Myocardial Ischemia/Reperfusion Damage and Links ER Stress and Oxidative Stress Signaling Pathways in the Heart.
RATIONALE: Endoplasmic reticulum (ER) stress causes the accumulation of misfolded proteins in the ER, activating the transcription …
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Activation of the ATF6 branch of the unfolded protein response in neurons improves stroke outcome.
Impaired function of the endoplasmic reticulum (ER stress) is a hallmark of many human diseases including stroke. To restore ER …
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CaMKIIδ subtypes differentially regulate infarct formation following ex vivo myocardial ischemia/reperfusion through NF-κB and TNF-α.
Deletion of Ca(2+)/calmodulin-dependent protein kinase II delta (CaMKIIδ) has been shown to protect against in vivo …
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Junctophilin-2 gene therapy rescues heart failure by normalizing RyR2-mediated Ca(2+) release.
BACKGROUND: Junctophilin-2 (JPH2) is the primary structural protein for the coupling of transverse (T)-tubule associated cardiac L-type …
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S100A4 protects the myocardium against ischemic stress.
BACKGROUND: Myocardial infarction is followed by cardiac dysfunction, cellular death, and ventricular remodeling, including tissue …
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Breaking down the COP9 Signalsome in the heart: how inactivating a protein ubiquitin ligase increases protein ubiquitylation and protects the heart.
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Hrd1 and ER-Associated Protein Degradation, ERAD, are Critical Elements of the Adaptive ER Stress Response in Cardiac Myocytes.
RATIONALE: Hydroxymethyl glutaryl-coenzyme A reductase degradation protein 1 (Hrd1) is an endoplasmic reticulum (ER)-transmembrane E3 …
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Finding the missing link between the unfolded protein response and O-GlcNAcylation in the heart.
The ER stress-inducible transcription factor, x-box binding protein 1 (XBP1), which enhances protein glycosylation in the endoplasmic …
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Roles for ATF6 and the sarco/endoplasmic reticulum protein quality control system in the heart.
The hypertrophic growth of cardiac myocytes is a highly dynamic process that underlies physiological and pathological adaptation of the …
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PRAS40 prevents development of diabetic cardiomyopathy and improves hepatic insulin sensitivity in obesity.
Diabetes is a multi-organ disease and diabetic cardiomyopathy can result in heart failure, which is a leading cause of morbidity and …
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Mechanistic target of rapamycin complex 2 protects the heart from ischemic damage.
BACKGROUND: The mechanistic target of rapamycin (mTOR) comprises 2 structurally distinct multiprotein complexes, mTOR complexes 1 and 2 …
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Classic studies of cultured cardiac myocyte hypertrophy: interview with a transformer.
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Pathological hypertrophy amelioration by PRAS40-mediated inhibition of mTORC1.
Mechanistic target of rapamycin complex 1 (mTORC1), necessary for cellular growth, is regulated by intracellular signaling mediating …
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Regulation of cardiac hypertrophic signaling by prolyl isomerase Pin1.
RATIONALE: Cardiac hypertrophy results from the complex interplay of differentially regulated cascades based on the phosphorylation …
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New concepts of endoplasmic reticulum function in the heart: programmed to conserve.
Secreted and membrane proteins play critical roles in myocardial health and disease. Studies in non-myocytes have shown that the …
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ATF6 [corrected] and thrombospondin 4: the dynamic duo of the adaptive endoplasmic reticulum stress response.
Thrombospondins are secreted, extracellular matrix (ECM) proteins that are upregulated in the heart and other tissues in response to …
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Roles for the sarco-/endoplasmic reticulum in cardiac myocyte contraction, protein synthesis, and protein quality control.
Although the function of the sarcoplasmic/endoplasmic reticulum (SR/ER) in cardiac contractile calcium handling is well established, …
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Limitation of individual folding resources in the ER leads to outcomes distinct from the unfolded protein response.
ER stress leads to upregulation of multiple folding and quality control components, known as the unfolded protein response (UPR). …
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Protein disulfide isomerase-associated 6 is an ATF6-inducible ER stress response protein that protects cardiac myocytes from ischemia/reperfusion-mediated cell death.
Proper folding of secreted and transmembrane proteins made in the rough endoplasmic reticulum (ER) requires oxygen for disulfide bond …
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Clarifying the cardiac proteasome paradox: protein quality control.
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Mesencephalic astrocyte-derived neurotrophic factor protects the heart from ischemic damage and is selectively secreted upon sarco/endoplasmic reticulum calcium depletion.
The endoplasmic reticulum (ER) stress protein mesencephalic astrocyte-derived neurotrophic factor (MANF) has been reported to protect …
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Regulation of microRNA expression in the heart by the ATF6 branch of the ER stress response.
A nodal regulator of endoplasmic reticulum stress is the transcription factor, ATF6, which is activated by ischemia and protects the …
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Functions for the cardiomyokine, MANF, in cardioprotection, hypertrophy and heart failure.
We define cardiomyokines as heart-derived secreted proteins that affect cardiovascular function via autocrine, paracrine and/or …
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The cardiokine story unfolds: ischemic stress-induced protein secretion in the heart.
Intercellular communication depends on many factors, including proteins released via the classical or non-classical secretory pathways, …
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PHLPP-1 negatively regulates Akt activity and survival in the heart.
RATIONALE: The recently discovered PHLPP-1 (PH domain leucine-rich repeat protein phosphatase-1) selectively dephosphorylates Akt at …
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Pim-1 kinase protects mitochondrial integrity in cardiomyocytes.
RATIONALE: Cardioprotective signaling mediates antiapoptotic actions through multiple mechanisms including maintenance of mitochondrial …
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Roles for endoplasmic reticulum-associated degradation and the novel endoplasmic reticulum stress response gene Derlin-3 in the ischemic heart.
RATIONALE: Stresses, such as ischemia, impair folding of nascent proteins in the rough endoplasmic reticulum (ER), activating the …
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Ischemia activates the ATF6 branch of the endoplasmic reticulum stress response.
Stresses that perturb the folding of nascent endoplasmic reticulum (ER) proteins activate the ER stress response. Upon ER stress, …
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The ATF6-Met[67]Val substitution is associated with increased plasma cholesterol levels.
OBJECTIVE: Activating transcription factor 6 (ATF6) is a sensor of the endoplasmic reticulum stress response and regulates expression …
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Kinetics of the translocation and phosphorylation of alphaB-crystallin in mouse heart mitochondria during ex vivo ischemia.
alphaB-crystallin (alphaBC) is a small heat shock protein expressed at high levels in the myocardium where it protects from …
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LPS-induced autophagy is mediated by oxidative signaling in cardiomyocytes and is associated with cytoprotection.
Bacterial endotoxin lipopolysaccharide (LPS) is responsible for the multiorgan dysfunction that characterizes septic shock and is …
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Mesencephalic astrocyte-derived neurotrophic factor is an ischemia-inducible secreted endoplasmic reticulum stress response protein in the heart.
The endoplasmic reticulum (ER) stress response (ERSR) is activated when folding of nascent proteins in the ER lumen is impeded. …
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Coordination of growth and endoplasmic reticulum stress signaling by regulator of calcineurin 1 (RCAN1), a novel ATF6-inducible gene.
Exposing cells to conditions that modulate growth can impair endoplasmic reticulum (ER) protein folding, leading to ER stress and …
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The role of the unfolded protein response in the heart.
The misfolding of nascent proteins, or the unfolding of proteins after synthesis is complete, can occur in response to numerous …
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Localization of phosphorylated alphaB-crystallin to heart mitochondria during ischemia-reperfusion.
The cytosolic small heat shock protein alphaB-crystallin (alphaBC) is a molecular chaperone expressed in large quantities in the heart, …
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Pim-1 regulates cardiomyocyte survival downstream of Akt.
The serine-threonine kinases Pim-1 and Akt regulate cellular proliferation and survival. Although Akt is known to be a crucial …
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Getting a G--RRP on regulated exocytosis in the heart.
A study by Rybkin et al. (see p. 527) substantially advances our understanding of regulated exocytois by specialized secretory cells, …
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Endoplasmic reticulum stress in the heart.
Over the last decade, it has become clear that the accumulation of misfolded proteins contributes to a number of neurodegenerative, …
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Effects of the isoform-specific characteristics of ATF6 alpha and ATF6 beta on endoplasmic reticulum stress response gene expression and cell viability.
The endoplasmic reticulum (ER)-transmembrane proteins, ATF6 alpha and ATF6 beta, are cleaved during the ER stress response (ERSR). The …
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Alterations in oxidative phosphorylation complex proteins in the hearts of transgenic mice that overexpress the p38 MAP kinase activator, MAP kinase kinase 6.
Ischemia-reperfusion (I/R) has critical consequences in the heart. Recent studies on the functions of I/R-activated kinases, such as …
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Activation of the unfolded protein response in infarcted mouse heart and hypoxic cultured cardiac myocytes.
Endoplasmic reticulum (ER) stresses that reduce ER protein folding activate the unfolded protein response (UPR). One effector of the …
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Endoplasmic reticulum stress gene induction and protection from ischemia/reperfusion injury in the hearts of transgenic mice with a tamoxifen-regulated form of ATF6.
Ischemia/reperfusion (I/R) affects the integrity of the endoplasmic reticulum (ER), the site of synthesis and folding of numerous …
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Atrial natriuretic peptide promotes cardiomyocyte survival by cGMP-dependent nuclear accumulation of zyxin and Akt.
This study delineates a mechanism for antiapoptotic signaling initiated by atrial natriuretic peptide (ANP) stimulation leading to …
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Activation of p38 has opposing effects on the proliferation and migration of endothelial cells.
Pathological conditions such as hypertension and hyperglycemia as well as abrasions following balloon angioplasty all lead to …
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Overexpression of mitogen-activated protein kinase kinase 6 in the heart improves functional recovery from ischemia in vitro and protects against myocardial infarction in vivo.
The mitogen-activated protein kinases (MAPK) have been the subject of many studies to identify signaling pathways that promote cell …
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Opposing roles for ATF6alpha and ATF6beta in endoplasmic reticulum stress response gene induction.
The endoplasmic reticulum (ER) transmembrane proteins, ATF6alpha and ATF6beta, are cleaved in response to ER stress, which can be …
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Roles for alphaB-crystallin and HSPB2 in protecting the myocardium from ischemia-reperfusion-induced damage in a KO mouse model.
Overexpression studies have shown that the small heat shock proteins (sHSP) protect the myocardium from ischemia-reperfusion …
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The MKK6-p38 MAPK pathway prolongs the cardiac contractile calcium transient, downregulates SERCA2, and activates NF-AT.
OBJECTIVE: Our goal was to determine if the MKK6-p38 MAPK pathway regulates cardiac intracellular calcium (Ca(2+)). We also tested if …
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MAP kinase kinase 6-p38 MAP kinase signaling cascade regulates cyclooxygenase-2 expression in cardiac myocytes in vitro and in vivo.
Cyclooxygenase-2 (COX-2) catalyzes the rate-limiting step in delayed prostaglandin biosynthesis. The purpose of this study was to …
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Factor associated with neutral sphingomyelinase activation and its role in cardiac cell death.
Generation of proapoptotic sphingolipids by neutral sphingomyelinase activation is an early response to hypoxia/reoxygenation (HR) in …
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Expression and functional characterization of SCaMPER: a sphingolipid-modulated calcium channel of cardiomyocytes.
Calcium channels are important in a variety of cellular events including muscle contraction, signaling, proliferation, and apoptosis. …
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Mimicking phosphorylation of alphaB-crystallin on serine-59 is necessary and sufficient to provide maximal protection of cardiac myocytes from apoptosis.
AlphaB-crystallin (alphaBC), a small heat shock protein expressed in high levels in the heart, is phosphorylated on Ser-19, 45, and 59 …
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Coordination of ATF6-mediated transcription and ATF6 degradation by a domain that is shared with the viral transcription factor, VP16.
ATF6 is a 670-amino acid endoplasmic reticulum (ER) transmembrane protein that is cleaved in response to ER stress. The resulting …
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Guanine nucleotide exchange factor-like factor (Rlf) induces gene expression and potentiates alpha 1-adrenergic receptor-induced transcriptional responses in neonatal rat ventricular myocytes.
Expression of constitutively active Ras (V12Ras) in cultured neonatal rat ventricular myocytes or targeted cardiac expression of V12Ras …
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Sarco/endoplasmic reticulum calcium ATPase-2 expression is regulated by ATF6 during the endoplasmic reticulum stress response: intracellular signaling of calcium stress in a cardiac myocyte model system.
The recently described transcription factor, ATF6, mediates the expression of proteins that compensate for potentially stressful …
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p38 MAPK regulates group IIa phospholipase A2 expression in interleukin-1beta -stimulated rat neonatal cardiomyocytes.
Group IIa phospholipase A(2) (GIIa PLA(2)) is released by some cells in response to interleukin-1beta. The purpose of this study was to …
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The cytoprotective effects of the glycoprotein 130 receptor-coupled cytokine, cardiotrophin-1, require activation of NF-kappa B.
Many cell types mount elaborate, compensatory responses to stress that enhance survival; however, the intracellular signals that govern …
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Ras reduces L-type calcium channel current in cardiac myocytes. Corrective effects of L-channels and SERCA2 on [Ca(2+)](i) regulation and cell morphology.
Heart failure is associated with dysregulation of intracellular calcium (Ca(2+)), reduction in myofibrils, and increased activation of …
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Expression and characterization of Edg-1 receptors in rat cardiomyocytes: calcium deregulation in response to sphingosine 1-phosphate.
Recent evidence indicates that sphingolipids are produced by the heart during hypoxic stress and by blood platelets during thrombus …
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p38 MAPK and NF-kappa B collaborate to induce interleukin-6 gene expression and release. Evidence for a cytoprotective autocrine signaling pathway in a cardiac myocyte model system.
In cardiac myocytes, the stimulation of p38 MAPK by the MAPKK, MKK6, activates the transcription factor, NF-kappaB, and protects cells …
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alpha B-crystallin gene induction and phosphorylation by MKK6-activated p38. A potential role for alpha B-crystallin as a target of the p38 branch of the cardiac stress response.
The MAPK kinase MKK6 selectively stimulates p38 MAPK and confers protection against stress-induced apoptosis in cardiac myocytes. …
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The Rho effector, PKN, regulates ANF gene transcription in cardiomyocytes through a serum response element.
The low-molecular-weight GTP-binding protein RhoA mediates hypertrophic growth and atrial natriuretic factor (ANF) gene expression in …
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LPS-induced TNF-alpha release from and apoptosis in rat cardiomyocytes: obligatory role for CD14 in mediating the LPS response.
The bacterial endotoxin lipopolysaccharide (LPS) contributes to the cardiovascular collapse and death observed in patients with sepsis. …
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The Raf-MEK-ERK cascade represents a common pathway for alteration of intracellular calcium by Ras and protein kinase C in cardiac myocytes.
Ras and protein kinase C (PKC), which regulate the Raf-MEK-ERK cascade, may participate in the development of cardiac hypertrophy, a …
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p38 Mitogen-activated protein kinase mediates the transcriptional induction of the atrial natriuretic factor gene through a serum response element. A potential role for the transcription factor ATF6.
In various cell types certain stresses can stimulate p38 mitogen-activated protein kinase (p38 MAPK), leading to the transcriptional …
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Beta1 integrins participate in the hypertrophic response of rat ventricular myocytes.
Multiple signaling pathways have been implicated in the hypertrophic response of ventricular myocytes, yet the importance of …
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MKK6 activates myocardial cell NF-kappaB and inhibits apoptosis in a p38 mitogen-activated protein kinase-dependent manner.
In cardiac myocytes the stimulation of p38 mitogen-activated protein kinase activates a hypertrophic growth program and the induction …
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A role for the p38 mitogen-activated protein kinase pathway in myocardial cell growth, sarcomeric organization, and cardiac-specific gene expression.
Three hallmark features of the cardiac hypertrophic growth program are increases in cell size, sarcomeric organization, and the …
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Collaborative roles for c-Jun N-terminal kinase, c-Jun, serum response factor, and Sp1 in calcium-regulated myocardial gene expression.
Electrical stimulation of contractions (pacing) of primary neonatal rat ventricular myocytes increases intracellular calcium and …
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Differential effects of protein kinase C, Ras, and Raf-1 kinase on the induction of the cardiac B-type natriuretic peptide gene through a critical promoter-proximal M-CAT element.
The cardiac genes for the A- and B-type natriuretic peptides (ANP and BNP) are coordinately induced by growth promoters, such as …
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Sphingosylphosphocholine modulates the ryanodine receptor/calcium-release channel of cardiac sarcoplasmic reticulum membranes.
Sphingosylphosphocholine (SPC) modulates Ca2+ release from isolated cardiac sarcoplasmic reticulum membranes; 50 microM SPC induces the …
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Tumor necrosis factor alpha-induced apoptosis in cardiac myocytes. Involvement of the sphingolipid signaling cascade in cardiac cell death.
In the present study, it was shown that physiologically relevant levels of the proinflammatory cytokine TNFalpha induced apoptosis in …
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Stabilization of the B-type natriuretic peptide mRNA in cardiac myocytes by alpha-adrenergic receptor activation: potential roles for protein kinase C and mitogen-activated protein kinase.
In cardiac myocytes, B-type natriuretic peptide (BNP) expression is induced with the rapid kinetics of a primary response gene. Like …
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Dissociation of p44 and p42 mitogen-activated protein kinase activation from receptor-induced hypertrophy in neonatal rat ventricular myocytes.
In response to hormones and mechanical stretch, neonatal rat ventricular myocytes exhibit a hypertrophic response that is characterized …
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Cardiotrophin-1 activates a distinct form of cardiac muscle cell hypertrophy. Assembly of sarcomeric units in series VIA gp130/leukemia inhibitory factor receptor-dependent pathways.
Cardiotrophin-1 (CT-1) was recently isolated by expression cloning based on its ability to induce an increase in cell size in neonatal …
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Involvement of multiple cis elements in basal- and alpha-adrenergic agonist-inducible atrial natriuretic factor transcription. Roles for serum response elements and an SP-1-like element.
In the present study, cis elements in the 5'-flanking sequence (FS) of the rat atrial natriuretic factor (ANF) gene involved in …
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TNF alpha receptor expression in rat cardiac myocytes: TNF alpha inhibition of L-type Ca2+ current and Ca2+ transients.
Tumor necrosis factor-alpha (TNF alpha) is a potentially powerful anti-neoplastic agent; however, its therapeutic usefulness is limited …
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Control of cardiac Ca2+ levels. Inhibitory actions of sphingosine on Ca2+ transients and L-type Ca2+ channel conductance.
The naturally occurring second messenger sphingosine (SPH) was examined for its ability to influence cardiac myocyte Ca2+ regulation. …
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Brain natriuretic peptide is induced by alpha 1-adrenergic agonists as a primary response gene in cultured rat cardiac myocytes.
To better understand the molecular basis for increased atrial natriuretic factor (ANF) and brain natriuretic peptide (BNP) expression …
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Regulation of rat brain natriuretic peptide transcription. A potential role for GATA-related transcription factors in myocardial cell gene expression.
GATA-binding proteins are transcription factors that regulate the stage- and tissue-specific expression of globin genes in cells of the …
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Involvement of cytoplasmic calcium and protein kinases in the regulation of atrial natriuretic factor secretion by contraction rate and endothelin.
To characterize the effects of the cellular events associated with contraction on atrial natriuretic factor (ANF) secretion, primary …
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Identification of the receptor subtype responsible for endothelin-mediated protein kinase C activation and atrial natriuretic factor secretion from atrial myocytes.
Endothelin-1 (ET-1) is a potent stimulator of atrial natriuretic factor (ANF) secretion from myocardial cells. In heart tissue there …
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Myocardial alpha-thrombin receptor activation induces hypertrophy and increases atrial natriuretic factor gene expression.
The protease, alpha-thrombin (alpha Th), affects myocardial cell contractility, a feature common among agents that induce hypertrophy. …
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Phenylephrine and endothelin differentially stimulate cardiac PI hydrolysis and ANF expression.
In ventricular myocytes, phenylephrine (PE) and endothelin (ET) stimulate phosphoinositide (PI) hydrolysis, cell growth, and expression …
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Regulated secretion of atrial natriuretic factor from cultured ventricular myocytes.
We have investigated endothelin (ET)-regulated secretion of atrial natriuretic factor (ANF) from primary neonatal rat ventricular …
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Induction of atrial natriuretic factor and myosin light chain-2 gene expression in cultured ventricular myocytes by electrical stimulation of contraction.
While hormonal stimuli and mechanical stretch can induced cardiac-specific gene expression and in some cases cellular hypertrophy, the …
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Gene expression and atrial natriuretic factor processing and secretion in cultured AT-1 cardiac myocytes.
BACKGROUND: Studies were carried out to characterize several biochemical features of cultured AT-1 cells. METHODS AND RESULTS: These …
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The cosecretional maturation of atrial natriuretic factor by primary atrial myocytes.
Cardiac myocytes store the 126-amino acid precursor of atrial natriuretic factor (pro-ANF), yet the mature, bioactive 28-amino acid …
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Protein kinase C and calmodulin kinase are required for endothelin-stimulated atrial natriuretic factor secretion from primary atrial myocytes.
Endothelin (ET), a potent stimulator of atrial natriuretic factor (ANF) secretion in atrial myocyte cultures, has been hypothesized to …
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Studies of ANF processing and secretion using a primary cardiocyte culture model.
In contrast to most other endocrine peptides ANF is stored in the heart as part of a larger prohormone, often called pro-ANF, yet is …
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The alpha-adrenergic stimulation of atrial natriuretic factor expression in cardiac myocytes requires calcium influx, protein kinase C, and calmodulin-regulated pathways.
It has been shown recently that alpha-adrenergic agonists can stimulate atrial natriuretic factor (ANF) expression in ventricular …
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Co-regulation of the atrial natriuretic factor and cardiac myosin light chain-2 genes during alpha-adrenergic stimulation of neonatal rat ventricular cells. Identification of cis sequences within an embryonic and a constitutive contractile protein gene which mediate inducible expression.
To study the mechanisms which mediate the transcriptional activation of cardiac genes during alpha adrenergic stimulation, the present …
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Endothelin induction of inositol phospholipid hydrolysis, sarcomere assembly, and cardiac gene expression in ventricular myocytes. A paracrine mechanism for myocardial cell hypertrophy.
The present study examined the effects of endothelin-1 on phosphoinositide hydrolysis, diacylglycerol formation, and the induction of …
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Rat pro-atrial natriuretic factor expression and post-translational processing in mouse corticotropic pituitary tumor cells.
Atrial natriuretic factor (ANF) is stored within atrial myocyte secretory granules as pro-ANF (ANF-(1-126] and is proteolytically …
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Calcium dependence of phenylephrine-, endothelin-, and potassium chloride-stimulated atrial natriuretic factor secretion from long term primary neonatal rat atrial cardiocytes.
Since calcium is involved in both excitation-secretion and excitation-contraction coupling, it was of interest to evaluate its …
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Chromatographic characterization of vasoactive intestinal polypeptide in guinea pig and rhesus monkey eyes.
Acid extracts of guinea pig and rhesus monkey anterior uvea, choroid and retina contain immunoreactive VIP. By reversed phase high …
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Regulation of atrial natriuretic factor-(99-126) secretion from neonatal rat primary atrial cultures by activators of protein kinases A and C.
Atrial natriuretic factor (ANF) is stored in atrial myocytes as a prohormone (ANF-(1-126] and is cosecretionally processed to the …
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Biochemical studies of soluble atrial natriuretic peptide (ANP) receptors from rat olfactory bulb and vascular smooth muscle cells.
  1. Aim. The biochemical characteristics of atrial natriuretic peptide receptors (ANP-R) derived from rat vascular smooth muscle (A-10 …
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Secretion of atrial natriuretic factor-(1-98) by primary cardiac myocytes.
Previous studies have demonstrated that primary cultures of cardiac myocytes maintained in a complete serum-free medium contain a …
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The secretion of atrial natriuretic factor-(99-126) by cultured cardiac myocytes is regulated by glucocorticoids.
Atrial natriuretic factor (ANF) is stored in mammalian atria primarily as ANF-(1-126), the precursor to the known circulating form of …
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Characterization of solubilized atrial natriuretic peptide receptors from rat olfactory bulb and A10 cultured smooth muscle cells.
Atrial natriuretic peptide (ANP) receptors from A10 cultured vascular smooth muscle cells (VSMC) and rat olfactory bulbs have been …
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The post-translational processing of rat pro-atrial natriuretic factor by primary atrial myocyte cultures.
Primary cultures of neonatal rat atrial myocytes were maintained in two different serum-free media for up to 25 days. Reversed-phase …
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The characterization of atrial natriuretic peptide (ANP) expression by primary cultures of atrial myocytes using an ANP-specific monoclonal antibody and an ANP messenger ribonucleic acid probe.
The biochemical and morphological characteristics of primary neonatal rat atrial myocytes were examined in order to establish a model …
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Characterization of the molecular forms of ANP released by perfused neonatal rat heart.
Although cultures of neonatal rat atria and ventricles have been widely used to study ANP biosynthesis and secretion, little is known …
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The conversion of atrial natriuretic peptide (ANP)-(1-126) to ANP-(99-126) by rat serum: contribution to ANP cleavage in isolated perfused rat hearts.
Atrial myocytes cultured for 7 days in serum-free medium secrete a 15K form of atrial natriuretic peptide (15K ANP), but isolated …
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The role of ascorbic acid in the biosynthesis of the neuroendocrine peptides alpha-MSH and TRH.
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Cross-linking of atrial natriuretic peptide to binding sites in rat olfactory bulb membranes.
Binding sites for 125I-atrial natriuretic peptide (ANP)2 in rat olfactory bulb membranes have been studied using pharmacological and …
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Autoradiographic localization and characterization of atrial natriuretic peptide binding sites in the rat central nervous system and adrenal gland.
Atrial natriuretic peptides (ANP) have recently been identified in both heart and CNS. These peptides possess potent natriuretic, …
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The characterization of the ascorbic acid-mediated alpha-amidation of alpha-melanotropin in cultured intermediate pituitary lobe cells.
Previous studies have shown that cultured rat intermediate pituitary lobe cells lose the ability to form ACTH-(1-13)NH2-related …
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Ascorbate transport by AtT20 mouse pituitary corticotropic tumor cells: uptake and secretion studies.
Ascorbate is an important cofactor in the biosynthesis of alpha-amidated endocrine and neural peptides. Peptidylglycine alpha-amidating …
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Immunoactive atrial natriuretic peptide in the rat eye: molecular forms in anterior uvea and retina.
Acid extracts of rat anterior uvea and retina contain immunoactive atrial natriuretic peptide. Gel filtration chromatography …
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Molecular forms of immunoactive atrial natriuretic peptide released from cultured rat atrial myocytes.
Primary cultures of atrial myocytes were prepared from newborn rats and maintained for 8 days in complete serum-free medium. The …
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Further characterization of the peptidyl alpha-amidating enzyme in rat anterior pituitary secretory granules.
In previous studies we have demonstrated a secretory granule-associated peptide alpha-amidation activity in rat anterior, intermediate, …
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Acetylation of alpha MSH and beta-endorphin by rat neurointermediate pituitary secretory granule-associated acetyltransferase.
ACTH(1-8) and ACTH(9-13)NH2 were used as potential enzyme inhibitors to begin examining the relationship between the acetylation of …
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Molecular forms of immunoactive atrial natriuretic peptide in the rat hypothalamus and atrium.
Acid extracts of rat hypothalamus and atrium were prepared by a procedure previously shown to minimize proteolytic degradation of …
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The alpha-amidation of alpha-melanocyte stimulating hormone in intermediate pituitary requires ascorbic acid.
Rat intermediate pituitary cells in primary culture display a time-dependent loss of the ability to produce COOH-terminally …
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Peptide alpha-amidation activity in mouse anterior pituitary AtT-20 cell granules: properties and secretion.
Mouse anterior pituitary corticotropic tumor cells (AtT-20/D-16v) were homogenized and subjected to subcellular fractionation. A …
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Characterization of a peptide alpha-amidation activity from rat anterior pituitary.
A secretory granule associated enzymatic activity from the rat anterior pituitary that can convert the synthetic peptide D-Tyr-Val-Gly …
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Bovine intermediate pituitary alpha-amidation enzyme: preliminary characterization.
A secretory granule-associated enzymatic activity that converts mono-[125I]-D-Tyr-Val-Gly into mono-[125I]-D-Tyr-Val-NH2 has been …
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Identification in pituitary tissue of a peptide alpha-amidation activity that acts on glycine-extended peptides and requires molecular oxygen, copper, and ascorbic acid.
An enzymatic activity capable of producing an alpha-amidated peptide product from its glycine-extended precursor has been identified in …
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Selective loss of alpha-melanotropin-amidating activity in primary cultures of rat intermediate pituitary cells.
The production of alpha-melanotropin (alpha-N-acetyl-ACTH(1-13)NH2 (alpha MSH) in rat intermediate pituitary was investigated using …
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Adrenocorticotropin(1-14)OH-related molecules in primary cultures of rat intermediate pituitary cells. Identification and role in the biosynthesis of alpha-melanotropin.
The structures of the altered alpha-melanotropin (alpha MSH or alpha-N-acetyl-ACTH(1-13)NH2)-related molecules produced by cultured rat …
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Characterization of the peptide acetyltransferase activity in bovine and rat intermediate pituitaries responsible for the acetylation of beta-endorphin and alpha-melanotropin.
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Acetylation of alpha-melanotropin and beta-endorphin in the rat intermediate pituitary. Subcellular localization.
The subcellular site of the further processing (NH2-terminal acetylation and COOH-terminal proteolysis) of beta-endorphin-sized …
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Subcellular fractionation studies on the post-translational processing of pro-adrenocorticotropic hormone/endorphin in rat intermediate pituitary.
The subcellular localization of the post-translational processing steps which occur in the conversion of pro-adrenocorticotropic …
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Adenylate energy charge in Escherichia coli CR341T28 and properties of heat-sensitive adenylate kinase.
Escherichia coli strain CR341T28 will not grow at temperatures above 34 degrees C in liquid medium, and the adenylate kinase of this …
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